Thursday, August 18, 2016

Difference between BOD and Bacteriological Incubators.

Bacteriological Incubators:


An ordinary incubator is generally based on ambient temperature for growth bacteria. It can have some basic heating arrangements. Bacteriological incubators are used to provide temperature range of 30-35°C for the growth of bacteria. Bacteriological incubators works at ambient temperature and it contains only heating mechanism. In these bacteriological incubators, if set temperature is in range of 30-35°C then they can only maintain their set temperature if surrounding environment temperature is below or in a range of 30-35°C. If surrounding temperature is higher then the incubator set temperature then there would be rise in display temperature of incubator because of high temperature of surrounding air and that air is used for the circulation in the chamber. Bacteriological incubator has no cooling mechanism to lower the display temperature. It fails in cold weather. If the room where it is stationed has right ambient temperature, it can still work.

BOD incubator:

Bod incubator has full control over the temperature as it is provided with both heating and cooling arrangements and provided +/- 0.5 0 C variation over the set temperature. It does not provide control over relative humudity. The environmental chamber provides control over temperature and relative humudity. BOD incubators are used to provide low temperature in range of 20-25°C for the growth of fungus and bacteria. BOD incubators have heating as well as cooling mechanism and they work independent of surrounding temperature. BOD incubators contains heating coils as we as compressor for cooling process. If incubator set at temperature of 20-25°C and surrounding temperature is higher than this set temperature then BOD incubator maintain the set temperature with the help of cooling mechanism and if surrounding temperature is low as compare to set temperature then heating would be generated with the help of heating coils. BOD incubators are also called low temperature incubators. These BOD incubators could be used as bacteriological incubator by changing the incubation set temperature requirements.

Mr. Khadga Bikram Angbuhang
Faculty: Microbilogy,
GoldenGate International College, Nepal
Research officer: LEAD Nepal
Past: Microbiologist, Chaudhary Group
Nepal

Monday, August 15, 2016

Determination of Dissolved Oxygen in Water by Titrimetric Method

Determination of Dissolved Oxygen in Water by Titrimetric Method

(Winkler method)

INTRODUCTION
The term Dissolved Oxygen is used to describe the amount of oxygen dissolved in a unit volume of water. It is a measure of the ability of water to sustain aquatic life. Dissolved oxygen (DO) is essential for the maintenance of healthy lakes and rivers. Any reduction in the amount of oxygen in a body of water, caused by a rise in temperature or by pollution, may mean that certain animals can no longer live there. The concentration of dissolved oxygen can therefore be taken as a measure of the ability of water to support living things. lf the oxygen concentration is less than half the maximum concentration then the water is of poor quality.........